Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: SHMT2 is up-regulated in OSCC cells. (a-b) The detection of SHMT2 mRNA and protein levels employed RT-qPCR and western blot in OSCC cell lines. *P < 0.05, ***P < 0.001 vs. HOK.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Quantitative RT-PCR, Western Blot

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 binds to SHMT2 and can be downregulated by SHMT2 silencing in OSCC cells. (a-b) The interaction between SHMT2 and ILF2 was found through the MINT and BioGRID databases. (c) ILF2 level in oral tumor tissue was analyzed using TNMplot database. (d-e) RT-qPCR and western blot analysis of ILF2 expression. **P < 0.01, ***P < 0.001 vs. HOK. (f) ILF2 expression in SHMT2-knockdown CAL-27 cells was detected with the use of western blot. ***P < 0.001 vs. si-NC. (g) The interaction of SHMT2 and ILF2 was identified using Co-IP.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Knockdown, Co-Immunoprecipitation Assay

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 upregulation reverses the impacts of SHMT2 knockdown on the occurrence of OSCC. (a-b) RT-qPCR and western blot analysis of the overexpression efficiency of ILF2. ***P < 0.001 vs. Oe-NC. (c) CCK-8 assay appraised cell viability in CAL-27 cells transfected with si-SHMT2 and Oe-ILF2. (d) Cell proliferation was identified utilizing colony formation assay in CAL-27 cells transfected with si-SHMT2 and Oe-ILF2. (e-f) TUNEL assay appraised cell apoptosis. (g-h) Western blot analysis of the expression of apoptosis-related factors. **P < 0.01, ***P < 0.001 vs. si-NC; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. si-SHMT2+ Oe-NC.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Over Expression, CCK-8 Assay, Transfection, Colony Assay, TUNEL Assay, Expressing

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 elevation restores the impacts of SHMT2 knockdown on OSCC cell migration, invasion and EMT. (a-b) Wound healing and transwell assays appraised cell migration and invasion. (c) Western blot analysis of MMP2 and MMP9 expression. (d) Western blot analysis of the expression of EMT-related factors. ***P < 0.001 vs. si-NC; ## P < 0.01, ### P < 0.001 vs. si-SHMT2+ Oe-NC.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Knockdown, Migration, Western Blot, Expressing